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ISOLASI DAN IDENTIFIKASI KAPANG DARI PETIS UDANG KOMERSIAL SERTA POTENSINYA SEBAGAI PENGHASIL KITINASE DAN PROTEASE

Murwantoko ; Murwantoko ; Murwantoko ; Murwantoko ; Murwantoko ; Ustadi ; Ustadi ; Ustadi ; Ustadi ; Ustadi ; Puspita, Indun Dewi ; Puspita, Indun Dewi ; Puspita, Indun Dewi ; Puspita, Indun Dewi ; Puspita, Indun Dewi ; Qistina, Amalina ; Qistina, Amalina ; Qistina, Amalina ; Qistina, Amalina ; Qistina, Amalina

Abstract:Abstract This study aimed to know and to identify the types of fungi on shrimp fermented product and to know the potential of these fungi in generating chitinases and proteases. Fungi isolation was conducted by spread method using potato dextrose agar medium (PDA). Fungi identification was done based on macroscopic, microscopic, and molecular observation. Molecular identification was conducted by DNA extraction based on Agusta (2009) method, amplification of 28S rDNA gene and DNA sequencing. The identification of chitinases and proteases production was done by identifying and measuring the chitinolitic index and proteolitic index on the clear zone created when the fungi placed on chitin agar and skim milk agar (SMA) medium. The results showed that the fungi was isolated from shrimp fermented product. The macroscopic, microscopic, and molecular identification results showed that PG1 and PG2 isolate were Aspergillus versicolor, PG3, PG4, and PS5 isolate were Aspergillus niger, and PS6 isolate were Alternaria cf. molesta. A chitinases activity and proteases activity of isolate PG2, PG3, and PS6 were tested. The PG3 isolate had a chitinase activity with the mean citinolytic index of 2,66 and The PG2, PG3, and PS6 isolates had a proteases activity with a low proteolytic index. The mean proteolytic index produced by PG2 isolate was 1,12; PG3 isolate was 1,11; and PS6 isolate was 1,14. All three identified fungi isolates did not have the potential to produce chitinases and proteases. Key words: chitinase activity, fungi, identification, protease activity, shrimp fermented product.

Intisari:Intisari Penelitian ini bertujuan untuk mengetahui dan mengidentifikasi jenis-jenis jamur yang tumbuh pada petis udang komersial dan mengetahui potensi jamur tersebut dalam menghasilkan kitinase dan protease. Isolasi jamur dilakukan dengan metode sebar menggunakan medium potato dextrose agar (PDA). Identifikasi jamur dilakukan berdasarkan pengamatan makroskopis, mikroskopis, dan molekuler. Identifikasi molekuler dilakukan dengan ekstraksi DNA menggunakan metode Agusta (2009), amplifikasi gen 28s rDNA dan sekuensing DNA. Potensi jamur tersebut dalam menghasilkan kitinase dan protease dilakukan dengan melihat dan mengukur indeks kitinolitik dan indeks proteolitik berdasarkan kenampakan zona bening yang dihasilkan jamur pada medium kitin agar dan skim milk agar (SMA). Hasil penelitian menunjukkan bahwa jamur berhasil diisolasi dari petis udang. Hasil identifikasi secara makroskopis, mikroskopis, dan molekuler menunjukkan bahwa isolat PG1 dan PG2 adalah Aspergillus versicolor, isolat PG3, PG4, dan PS5 adalah Aspergillus niger, dan isolat PS6 adalah Alternaria cf. molesta. Dilakukan uji aktivitas kitinase dan protease isolat PG2, PG3 dan PS6. Isolat PG3 memiliki aktivitas kitinase dengan rata-rata indeks kitinolitik 2,66 dan isolat PG2, PG3, dan PS6 memiliki aktivitas protease dengan indeks proteolitik yang rendah. Rata-rata indeks proteolitik yang dihasilkan isolat PG2 adalah 1,12; isolat PG3 adalah 1,11; dan isolat PG6 adalah 1,14. Ketiga isolat jamur yang telah teridentifikasi tidak berpotensi dalam menghasilkan kitinase dan protease. Kata kunci : aktivitas kitinase, aktivitas protease, identifikasi, jamur, petis udang.===Abstract This study aimed to know and to identify the types of fungi on shrimp fermented product and to know the potential of these fungi in generating chitinases and proteases. Fungi isolation was conducted by spread method using potato dextrose agar medium (PDA). Fungi identification was done based on macroscopic, microscopic, and molecular observation. Molecular identification was conducted by DNA extraction based on Agusta (2009) method, amplification of 28S rDNA gene and DNA sequencing. The identification of chitinases and proteases production was done by identifying and measuring the chitinolitic index and proteolitic index on the clear zone created when the fungi placed on chitin agar and skim milk agar (SMA) medium. The results showed that the fungi was isolated from shrimp fermented product. The macroscopic, microscopic, and molecular identification results showed that PG1 and PG2 isolate were Aspergillus versicolor, PG3, PG4, and PS5 isolate were Aspergillus niger, and PS6 isolate were Alternaria cf. molesta. A chitinases activity and proteases activity of isolate PG2, PG3, and PS6 were tested. The PG3 isolate had a chitinase activity with the mean citinolytic index of 2,66 and The PG2, PG3, and PS6 isolates had a proteases activity with a low proteolytic index. The mean proteolytic index produced by PG2 isolate was 1,12; PG3 isolate was 1,11; and PS6 isolate was 1,14. All three identified fungi isolates did not have the potential to produce chitinases and proteases. Key words: chitinase activity, fungi, identification, protease activity, shrimp fermented product.

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Penerbit : Departemen Perikanan Fakultas Pertanian UGM, 2017
Tahun 2017
Deskripsi xiii, 76 hlm .; ill .: 12,5 cm
No Klasifikasi -
Bahasa Indonesian
Jenis Penelitian Skripsi
Subyek jamur
Kata Kunci aktivitas kitinase, aktivitas protease, identifikasi, jamur, petis udang.
Jumlah Eksemplar 1
Barcode No. Inventaris Lokasi
  -   181026FPN   Perpustakaan Fakultas Pertanian